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Schematics, Diagrams, Circuits, and Given Data: RB = 10 k ; RC = 375 ; VBB = 21 V; VCC = 15 V; V = 06 V The collector characteristic curves are shown in Figure 913 Assumptions: Assume that the BE junction resistance is negligible when compared to the base resistance Assume that each voltage and current can be represented by the superposition of a DC (quiescent) value and an AC component For example: v0 (t) = V0Q + V0 (t) Analysis:

vBE (t)

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changes are replacements, additions, or deletions. A second point mutation in the same gene can result either in a double mutation, reversion to the original, or intragenic suppression. In this case, intragenic suppression is illustrated by the addition of one base followed by the nearby deletion of a different base.

1 DC operating point On the assumption the BE junction resistance is much smaller than RB , we can state that the junction voltage is constant: vBE (t) = VBEQ = V , and plays a role only in the DC circuit The DC equivalent circuit for the base is shown in Figure 918 and described by the equation VBB = RB IBQ + VBEQ from which we compute the quiescent base current: IBQ = VBB VBEQ VBB V 21 06 = 150 A = = RB RB 10,000

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21 V

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To determine the DC operating point, we write the load line equation for the collector circuit: VCE = VCC RC IC = 15 375IC The load line is shown in Figure 919 The intersection of the load line with the 150 A base curve is the DC operating or quiescent point of the transistor ampli er, de ned below by the three values: VCEQ = 72 V; ICQ = 22 mA; IBQ = 150 A

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or subtract a base are, potentially, the most devastating in their effects on the cell or organism because they change the reading frame of a gene from the site of mutation onward ( g. 12.13). A frameshift mutation causes two problems. First, all the codons from the frameshift on will be different and thus yield (most probably) a useless protein. Second, stop-signal information will be misread. One of the new codons may be a nonsense codon, which causes translation to stop prematurely. Or, if the translation apparatus reaches the original nonsense codon, it is no longer recognized as such because it is in a different reading frame, and therefore, the translation process continues beyond the end of the gene.

190 A

286 22 153 0

that substitutes the AACCCT sequence for the original AAACCC. These sequences, when transcribed (UUGGGA, UUUGGG), are codons for leucine-glycine and phenylalanine-glycine, respectively. Intragenic suppression occurs whether the new codons are for different amino acids or the same amino acids, as long as the phenotype of the organism is reverted approximately to the original. Suppressed mutations can be distinguished from true back mutations either by subtle differences in phenotype, by genetic crosses, by changes in the amino acid sequence of a protein, or by DNA sequencing.

15 VCE (V)

A class of mutants that has been very useful to geneticists is the conditional-lethal mutant, a mutant that is lethal under one set of circumstances but not under another set. Nutritional-requirement mutants are good examples (see chapter 7). Temperature-sensitive mutants are conditional-lethal mutants that have made it possible for geneticists to work with genes that control vital functions of the cell, such as DNA synthesis. Many temperature-sensitive mutants are completely normal at 25 C but cannot synthesize DNA at 42 C. Presumably, temperature-sensitive mutations result in enzymes with amino acid substitutions that cause protein denaturation to occur at temperatures above normal. Thus, the enzyme has normal function at 25 C, the permissive temperature, but is nonfunctional at 42 C, the restrictive temperature.

2 AC current gain To determine the current gain, we resort, again, to the collector curves Figure 919 indicates that if we consider the values corresponding to base currents of 190 and 110 A, the collector will see currents of 286 and 153 mA,

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convert tiff to pdf c# itextsharp

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convert tiff to pdf c# itextsharp

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