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Figure 3.6 To add the picture, drag an NSImageView object from the palette window and resize it to fill the entire window.

140 199

200

1 Give 75 g of glucose dissolved in 300 mL of water after an overnight fast in persons who have been receiving at least 150 200 g of carbohydrate daily for 3 days before the test 2 A fasting plasma glucose 126 mg/dL is diagnostic of diabetes if confirmed on a subsequent day

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Mistakes happen It s a simple fact of life, and it s true in programming, too Even the most experienced programmers make mistakes, sometimes the same mistakes over and over You ve probably made a few as you ve gone through the exercises in this book Mistakes are normal, and they are easy to make, especially in a programming language such as C#; we even have a special word for programming mistakes: bugs You ve probably noticed that the compiler (that s Visual Studio or C# Express) catches a lot of your bugs, and tells you what s wrong It s even right a lot of the time, although it s certainly not perfect The more you learn, and the more you have the basics of the language down, the more complicated your mistakes become.

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Immunoassays that use an antibody to the glycated amino terminus of globin do not recognize the terminus of the globin of hemoglobin F Thus, in patients with high levels of hemoglobin F, immunoassays give falsely low values of HbA1c Cation-exchange chromatography separates hemoglobin species by charge differences Hemoglobin variants that co-elute with HbA1c can lead to an overestimation of the HbA1c value Chemically modified derivatives of hemoglobin such as carbamoylation (in renal failure) or acetylation (highdose aspirin therapy) can similarly co-elute with HbA1c by some assay methods Any condition that shortens erythrocyte survival or decreases mean erythrocyte age (eg, recovery from acute blood loss, hemolytic anemia) will falsely lower HbA1c irrespective of the assay method used Alternative methods such as fructosamine (see below) should be considered for these patients Vitamins C and E are reported to falsely lower test results possibly by inhibiting glycation of hemoglobin c Serum fructosamine Serum fructosamine is formed by nonenzymatic glycosylation of serum proteins (predominantly albumin) Since serum albumin has a much shorter half-life than hemoglobin, serum fructosamine generally reflects the state of glycemic control for only the preceding 1 2 weeks Reductions in serum albumin (eg, nephrotic state or hepatic disease) will lower the serum fructosamine value When abnormal hemoglobins or hemolytic states affect the interpretation of glycohemoglobin or when a narrower time frame is required, such as for ascertaining glycemic control at the time of conception in a diabetic woman who has recently become pregnant, serum fructosamine assays offer some advantage Normal values vary in relation to the serum albumin concentration and are 15 24 mmol/L when the serum albumin level is 5 g/dL d Self-monitoring of blood glucose Capillary blood glucose measurements performed by patients themselves, as outpatients, are extremely useful In type 1 patients in whom tight metabolic control is attempted, they are indispensable There are several paper strip (glucose oxidase, glucose dehydrogenase, or hexokinase) methods for measuring glucose on capillary blood samples A reflectance photometer or an amperometric system is then used to measure the reaction that takes place on the reagent strip A large number of blood glucose meters are now available All are accurate, but they vary with regard to speed, convenience, size of blood samples required, and cost Popular models include those manufactured by LifeScan (One Touch), Bayer Corporation (Ascencia), Roche Diagnostics (Accu-Chek), Abbott Laboratories (Precision, FreeStyle), and Home Diagnostics (Prestige) These blood glucose meters are relatively inexpensive, ranging from $50 to $100 each Test strips remain a major expense, costing $50 to$75 apiece Each glucose meter comes with a lancet device and disposable 26- to 33-gauge lancets Some meters have been approved for measuring glucose in blood samples obtained at alternative sites such as the forearm and thigh There is, however, a 5- to 20-minute lag in the glucose response on the arm with respect to the glucose response on the finger Forearm blood glucose measurements could therefore result in a delay in detection of rapidly developing hypoglycemia.

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