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Watson and Crick originally suggested that mutation could occur spontaneously during DNA replication if pairing errors occurred. If a base of the DNA underwent a proton shift into one of its rare tautomeric forms (tautomeric shift) during the replication process, an inappropriate pairing of bases would occur. Normally, adenine and cytosine are in the amino (NH2) form. Their tautomeric shifts are to the imino (NH) form. Similarly, guanine and thymine go from a keto (C O) form to an enol (COH) form ( g. 12.14). Table 12.4 shows the new

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and depths of cut, it can be used to work on brittle materials such as ceramics and glass in a ductile fashion (chip removal by ductile shearing of material, as in metal cutting [2] [3]) In some cases, the surface finish obtained with precision grinding is so good that polishing is unnecessary The grinding process has the advantage over polishing of having higher removal rates and the ability to remove vastly different amounts of material from small areas Thus, the grinding operation is particularly suited to produce especially small complex shapes in materials that cannot be diamond turned

Many issues remain to be considered before we can think of designing and analyzing a practical transistor ampli er It is extremely important that you master this example before studying the remainder of the section

The McGraw Hill Companies, 2001

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analyzed in this example is available in the CD-ROM that accompanies the book Run the simulation to see the effect of the negative voltage gain on the output signal waveform

In discussing the DC biasing procedure for the BJT, we pointed out that the simple circuit of Figure 912 would not be a practical one to use in an application

base pairings that would occur following tautomeric shifts of the DNA bases. Figure 12.15 illustrates the molecular structure of one of these tautomeric pairings. During DNA replication, a tautomeric shift in either the incoming base (substrate transition) or the base already in the strand (template transition) results in mispairing. The mispairing will be permanent and result in a new base pair after an additional round of DNA replication. The original strand is unchanged ( g. 12.16). In the example in gure 12.16, the replacement of one base pair maintains the same purine-pyrimidine relationship: AT is replaced by GC and GC by AT. In both examples, a purine-pyrimidine combination is replaced by a purine-pyrimidine combination. (Or, more speci cally, a purine replaces another purine: guanine replaces

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circuit In fact, the more realistic circuit of Example 94 is also not a practical biasing circuit The reasons for this statement are that two different supplies are required (VCC and VBB ) a requirement that is not very practical and that the resulting DC bias (operating) point is not very stable This latter point may be made clearer by pointing out that the location of the operating point could vary signi cantly if, say, the current gain of the transistor, , were to vary from device to device A circuit that provides great improvement on both counts is shown in Figure 920 Observe, rst, that the voltage supply, VCC , appears across the pair of resistors R1 and R2 , and that therefore the base terminal for the transistor will see the Th venin equivalent circuit composed of the equivalent voltage source, e VBB = R2 VCC R1 + R 2 R2 (96)

Grinding wheels are made of two materials, abrasive grains and a bonding material They are produced by mixing the appropriate grain size of the abrasive with the required bonding material and pressed into shape The abrasive grains do the actual cutting, and the bonding material holds the grains together and supports them while they cut The cutting action of a grinding wheel is dependent on the bonding material, the abrasive type, grain size (grit size), wheel grade and the wheel structure Selection of the right combination of these features is therefore essential for obtaining an optimum solution for different grinding tasks

and of the equivalent resistance, RB = R1 (97)

Cytosine Substrate transition tautomerization of incoming adenine Figure 12.16 Tautomeric shifts result in transition mutations.

Figure 921(b) shows a redrawn DC bias circuit that makes this observation more evident The circuit to the left of the dashed line in Figure 921(a) is represented in Figure 921(b) by the equivalent circuit composed of VBB and RB

Adenine (amino)

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